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KMID : 0364219700130030085
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Korean Journal of Zoology
1970 Volume.13 No. 3 p.85 ~ p.93
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Studies on the Metabolism-Independent Calium Binding of the Rat Liver Mitochondria
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Kang Shin-Sung
Ha Doo-Bong
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Abstract
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Measurements were made of the $Ca^++$ uptake, oxygen consumption and ATPase activity of mitochondria extracted from the rat liver in sucrose-tris chloride medium. $Ca^++$ binding of mitochondria was not affected by the incubation temperature in the range of $0^\\circ - 37^\\circ C$. Succinate did not increase the amount of $Ca^++$ bound while it increased oxygen consumption highly. The presence of ATP in the incubation medium did not enhance the $Ca^++$ uptake either. Therefore, it is concluded that the initial binding of $Ca^++$ is independent on metabolism.
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